Whole genome sequencing of Vibrio fischeri strain ES114 - SRA

SRX23668392: Whole genome sequencing of Vibrio fischeri strain ES114
1 ILLUMINA (NextSeq 2000) run: 2.6M spots, 391.6M bases, 137.3Mb downloads

Design: Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.

Submitted by: University of Georgia

Study: Vibrio fischeri D-alanine suppressor generation and characterization

PRJNA1078398 • SRP490497 • All experiments • All runs

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This study used D-alanine auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-alanine. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.

Sample:

SAMN39999377 • SRS20499470 • All experiments • All runs

Organism: Aliivibrio fischeri

Library:

Name: ES114 (2023)

Instrument: NextSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 2.6M spots, 391.6M bases, 137.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR28016264	2,636,178	391.6M	137.3Mb	2024-02-20

ID:: 31951809

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